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KMID : 0900919930170030243
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Korean journal of Animal Reproduction
1993 Volume.17 No. 3 p.243 ~ p.248
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Development of Bovine IVM/IVF Ebmryos Cultured in TCM-199 and Synthetic Oviduct Fluid(SOF) Medium with without Co-culture System
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Yang Boo-Keun
Park Choon-Keun
Kim Jong-Bok
Cheong Hee-Tae
Kim Choung-Ik
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Abstract
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Bovine embryos at 2-to 8-cell produced by in vitro matured/in vitro fertilized(IVM/IVF) were cultured in TCM 199 or Synthetic oviduct fluid(SOF) with 10% fetal bovine serum(FBS) or cocultured with cumulus or bovine oviduct epithelial cell(BOEC) in TCM-199 or SOF medium. In experiment 1, the proportions of embryos developed to morula and blastocysts stages in TCM 199 medium were higher when they were co-cultured with cumulus cell(29%) or BOEC(33%) than that of TCM 199 with 10% FBS(12%, P<0.01). In experiment 2, embryos deived from IVM/IVF were cultured in SOF with 10% FBS or cocultured with cumulus cell or BOEC in same medium. The higher development rates of IVM/IVF embryos developed beyond morula stages were obtained in cumulus cell co-culture group(39%) than those of BOEC group(26%) and SOF with 10% FBS group(17%, P<0.01). The present results indicated that the early development of IVM/IVF embryos can be maintained efficienty in SOF with cumulus cell co-culture.
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KEYWORD
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